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當(dāng)前位置:首頁資料下載Human dsDNA IgG kit 人雙鏈DNA 抗體檢測試劑盒說明書

Human dsDNA IgG kit 人雙鏈DNA 抗體檢測試劑盒說明書

發(fā)布時(shí)間:2023/8/11點(diǎn)擊次數(shù):781

Human anti-dsDNA IgG High Sensitivity ELISA Kit, 96 tests, Quantitative

Anti-ds-DNA IgG ELISA kit is based on binding of ds-DNA antibody (IgG) from serum samples to native ds-DNA immobilized on microtiter wells. After a washing step, goat anti-human IgG-HRP conjugate is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (color) is directly proportional to the amount of ds-DNA IgG present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450 nm and the concentration of anti-DNA IgG in samples is calculated on the basis of the absorbance of the negative, positive, and, calibrator standards..




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